![]() RNA-Seq results revealed changes in gene expression after HERV-K env KD, including RAS and TP53. Importantly, the in vitro and in vivo growth rates of three pancreatic cancer cell lines were significantly reduced after HERV-K KD by shRNA targeting HERV-K env, and there was reduced metastasis to lung after treatment. HERV-K viral RNA levels and anti-HERV-K antibody titers were significantly higher in pancreatic cancer patient sera ( N = 106) than in normal donor sera ( N = 40). Reverse transcriptase activity and virus-like particles were observed in culture media supernatant obtained from Panc-1 and Panc-2 cells. A new HERV-K splice variant was discovered in several pancreatic cancer cell lines. Results: HERV-K env expression was detected in seven pancreatic cancer cell lines and in 80% of pancreatic cancer patient biopsies, but not in two normal pancreatic cell lines or uninvolved normal tissues. Purpose: We investigated the role of the human endogenous retrovirus type K (HERV-K) envelope ( env) gene in pancreatic cancer.Įxperimental Design: shRNA was employed to knockdown (KD) the expression of HERV-K in pancreatic cancer cells.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |